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complementary dna cdna libraries (Roche)

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Roche complementary dna cdna libraries
Complementary Dna Cdna Libraries, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/complementary+dna+libraries/HyperPrep/pmc12506486-27-2-31
Average 99 stars, based on 5 article reviews

complementary dna cdna libraries - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Transcriptomic Architecture of Weak Versus Strong Contextual Fear Extinction Learning Uncovers Extinction-Suppressive Role of TIA1
Article Snippet: To generate complementary DNA (cDNA) libraries, ∼1 μg of total RNA per sample was enriched for polyA RNA and converted into libraries using the KAPA mRNA HyperPrep Kit for Illumina Platforms (KAPA Biosystems).

Article Title: Identification of new genes in Sinorhizobium meliloti using the Genome Sequencer FLX system.
Article Snippet: 1 ug of cDNA of each sample was size selected (>100 bp) using Roche's GS FLX library Preparation Guide recommendations (no nebulization was necessary due to the size range of the cDNA GS FLX library Preparation Guide), and a single stranded library was created.

DNA Library Preparation:

Article Title: Differential regulation of gene co-expression modules in muscles and liver of preterm newborns
Article Snippet: .. Complementary DNA library preparation KAPA RNA HyperPrep Kit with RiboErase KK8560 (F. Hoffmann-La Roche, Basel, Switzerland) was used for RNA-Seq library preparation with 1 μg RNA per sample. .. Sequencing was performed using NovaSeq6000 (Illumina, San Diego, CA, United States).

cDNA Library Assay:

Article Title: Peptide fingerprinting of the neurotoxic fractions isolated from the secretions of sea anemones Stichodactyla helianthus and Bunodosoma granulifera. New members of the APETx-like family identified by a 454 pyrosequencing approach.
Article Snippet: Also, the total RNA quantity was easured using Quant-iTTM RNA BR Assay Kit in Qubit® 2.0 Flurometer. cDNA was synthesized using Roche double-stranded DNA synthesis kit (Roche Applied Sciences, USA) from total RNA ith oligo (dT) 20 primer. .. A cDNA library was prepared using cDNA apid library preparation method kit (Roche Applied Science, GS unior Titanium Series, USA) according to manufacturer’s instrucion. ..

Synthesized:

Article Title: Reduced adult stem cell fate specification led to eye reduction in cave planarians
Article Snippet: .. Total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer’s instructions. cDNA-sequencing library was synthesized using stranded KAPA mRNA HyperPrep (Roche) and Kapa Dual-Indexed Adapter kit Illumina Platforms following the manufacturer’s protocol. .. Libraries were sequenced on an Illumina HiSeq 3000/4000 with 150 paired-end reads for an average sequencing depth of 20 million reads per sample.

Article Title: Reduced adult stem cell fate specification led to eye reduction in cave planarians.
Article Snippet: .. Total RNA was extracted using TRIzol (Invitrogen) according to the manufacturer’s instructions. cDNA-sequencing library was synthesized using stranded KAPA mRNA HyperPrep (Roche) and Kapa Dual-Indexed Adapter kit Illumina Platforms following themanufacturer’s protocol. .. Libraries were sequenced on an Illumina HiSeq 3000/4000 with 150 paired-end reads for an average sequencing depth of 20 million reads per sample.